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| Ferreira,Catia S. M.; Missailidis,Sotiris. |
| Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for a variety of applications. These range from inhibition of receptors and enzymes to the identification of small molecules in sensor applications, and from the development of targeted therapeutic to the design of novel diagnostic and imaging agents. Furthermore, aptamers have been designed for targets that cover a wide range of diseases, from HIV to tropical diseases, cancer and inflammation. Their easy development and flexibility of use and manipulation, offers further potential. In this paper we review their selection and consider some of the recent applications of aptamers in the design of radiopharmaceuticals for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aptamers; Radiopharmaceuticals; SELEX; Radiolabeling; Medical imaging. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600008 |
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| Shirmardi,SP; Shamsaei,M; Gandomkar,M; Saniei,E; Ghannadi,M; Zare,A. |
| Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesobuthus eupeus; Venom; Purification; Chromatography; Radiolabeling; Biodistribution. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015 |
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| Dias,Carla Roberta; Marczewski,Barbara; Moraes,Vanessa; Barboza,Marycel Figols de; Osso Junior,João Alberto. |
| Monoclonal antibodies (Mabs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in the practice of nuclear medicine. The ior egf/r3 (Centis, Cuba) is a murine monoclonal antibody against epidermal growth factor receptor (EGF-R) and has been widely used in the radioimmunodiagnosis of tumors of epithelial origin. Labeled with 99mTc, its main application in Nuclear Medicine is the follow up, detection and evaluation of tumor recurrences. The objective of this work is to describe the preparation of a lyophilized formulation (kit) for radiolabeling the Mab ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, image studies and stability of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Radiolabeling; Technetium; Monoclonal antibodies; Nuclear medicine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004 |
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| Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Olej,Beni; Rocha,Emely Kazan; Arnobio,Adriano; Bernardo-Filho,Mário; Caldas,Luiz Querino de Araújo; Honeycut,Hayden. |
| The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Uncaria tomentosa; Technetium-99m; Radiolabeling; Blood constituents. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024 |
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